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NOD /ShiLtJ Feederless ES Cell Line

Embryonic stem cell line from the non-obese diabetic (NOD) strain of Mus musculus (lab mouse). This ES cell line has a pure NOD genetic background and does not require feeder cells for propagation.

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Applications

Direct generation of animals in the NOD background, without the need to propagate polygenic animals through multiple generations

Unique features

Only available NOD ES cell line with feeder cell independent propagation
Robust cell line that enables straightforward maintenance and manipulation

Research area

Immunodeficiency; diabetes; cancer biology

Alternate reagents

While alternate reagents are available, they have drawbacks described below:

NOD/129 F1 hybrid ES cells, not pure genetic background, not feederless. Learn more.
NOD ES cells that require feeder cells for propagation. Learn more.
Feeder dependent NOD line, not robust, poor propagation. Learn more.
Feeder dependent, from different NOD substrain. Learn more.
Feeder dependent NOD ES cells. Learn more.

Publications/ references

None

Other sources of validation

International Society for Stem Cell Research Annual meeting (ISSCR 2020) – Boston/virtual meeting June 2020

Principal investigator

Kathleen J Millen, PhD

Learn more

To learn more about this cell line, please email Kamya Rajaram.

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